Conjugation

Part:BBa_K640005

Designed by: Emily Hicks   Group: iGEM11_Calgary   (2011-09-26)

Device for conjugating plasmids from E. coli to pseudomonas

This composite part contains the J01003 oriT previously submitted by iGEM_Berkeley, as well as an ori1600 part, a replication origin for pseudomonas (BBa_K640003). This part is in a kanamycin resistance vector to select for positive conjugation results. This construct allows for the conjugation of plasmids from E. Coli to pseudomonas and vice-versa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 499
    Illegal NgoMIV site found at 509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 401


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Categories
Parameters
None